Application of CRISPR/Cas System-based Biosensors in Detection of Foodborne Pathogens

Abstract:

The CRISPR/Cas system can recognize foreign DNA, cut the target, and block or silence the expression of the foreign gene. Due to its precise targeting function, the CRISPR/Cas system has been developed as a highly efficient gene editing tool. With high specificity and operability, the CRISPR/Cas system has been combined with biosensors to improve the detection specificity and efficiency of the sensors. This review introduces the characteristics and applications of CRISPR/Cas system, analyzes the latest progress in the application of CRISPR/Cas system-based biosensors in the detection of foodborne pathogens, and summarizes and prospects the application of such biosensors.