Research Progress of Lipase Enzyme Activity Detection Methods

Abstract:

As key biocatalysts, lipases play essential roles in catalyzing the hydrolysis of triglycerides and the esterification of fatty acids. Therefore, accurate measurement of lipases enzyme activity is crucial. Traditional methods, such as plate assay and titration, indirectly assess lipase enzyme activity by measuring the fatty acids produced from the substrate catalyzed by lipases. Methods like the copper-soap colorimetric assay, p-nitrophenol assay, and probe colorimetric assay determine lipase enzyme activity based on changes in the color of the reaction products. Recently developed methods, such as liquid foam detection, are limited to qualitative analysis, whereas the fluorescence probe and biosensor-based detection methods offer higher sensitivity, making them more suitable for measuring lipase enzyme activity in complex media. The author provides a brief overview of the principles, limitations applicable conditions, and current developments of various lipase enzyme activity detection methods, along with a comprehensive comparison and summary, aiming to offer a reference for future research on lipase enzyme activity detection.