Rapid Determination of Vitamin B1 in Infant Formula Milk Powder by Fluorescent Microsphere Immunochromatographic Assay

Abstract:

[Objective] A fluorescent immunochromatographic assay was established based on vitamin B₁ monoclonal antibody to measure the vitamin B₁ in infant formula milk powder.[Method] The performance of vitamin B₁ monoclonal antibody was evaluated. On this basis, a highly sensitive fluorescent immunochromatographic test strip was developed.[Result] The vitamin B₁ monoclonal antibody showed high sensitivity, affinity, and specificity. The fluorescent immunochromatographic test strip showed a visual limit of detection (vLOD) of 10 μg/kg, a cut-off value of 200 μg/kg, and a calculated limit of detection (cLOD) of 2.056 μg/kg. In addition, the recovery rate of this method was 97.3%~115.4%. The test results of the established method were consistent with the liquid chromatography-tandem mass spectrometry (LC-MS/MS) results, with the relative standard deviation (RSD) less than 10%, which indicated good accuracy and stability.[Conclusion] The fluorescent immunochromatographic assay can be used for the rapid detection of actual infant formula milk powder samples. It does not require large-scale instruments or equipment and is portable, thus being of great significance for the rapid screening of a large number of samples.