Recombinant Pichia pastoris Fermentation for Biosynthesis of the UV Resistant Compound Gadusol

Abstract:

[Objective]This study aims to employ synthetic biology and metabolic engineering techniques to engineer Pichia pastoris (P. pastoris) for the efficient heterologous synthesis of the UV resistant compound gadusol.[Method]On the basis of a bioinformatic analysis and codon optimization of the genes involved in the gadusol biosynthetic pathway in Danio rerio, the gadusol fermentation pathway was constructed in P. pastoris via strategies such as promoter engineering, metabolic engineering, and fermentation optimization.[Result]A high-purity gadusol standard was prepared through optimized extraction and chromatographic separation. The gadusol yield in the recombinant strain P. pastoris HME reached 105 mg/L. By screening of gene expression promoters and optimization of the fermentation medium components, the engineered strain, P. pastoris GME, achieved a gadusol yield of approximately 175 mg/L.[Conclusion]This study successfully constructed the strain P. pastoris GME capable of efficiently synthesizing gadusol, providing support and a reference for accelerating the industrial production of gadusol by fermentation and the research on the application of this compound in the food and cosmetic industries.