Abstract:As a crucial part of epigenetics, DNA methylation plays an indispensable role in various physiological processes in bacteria. Here, we compared the metabonomics of DNA methylation of mutant strain Lactobacillus casei (L. casei) Zhang ΔpglX and wild strain L. casei Zhang in the early logarithmic growth phase(4 h) using ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) non-targeted metabolomics technology. A total of twenty-three significantly different metabolites were identified(P <0.05), primarily enriched in pathways such as aminoacyl-tRNA biosynthesis, un-saturate fatty acids biosynthesis, D-glutamine and D-glutamate metabolism, and galactose metabolism. The results suggest that DNA methylation phenotype may affect the synthesis and utilization of amino acids in bacterial strains during early logarithmic growth. On the other hand, DNA methylation phenotypes may modulate the composition of cellular membrane fatty acids, altering membrane permeability and fluidity.
