PhrG-RapG-DegU Quorum Sensing Regulates Menaquinone-7 Production in Bacillus subtilis

Abstract:

Objective The effects of the transcription regulation system on menaquinone-7 (MK-7) production in Bacillus subtilis (B. subtilis) 168 were studied by excavating transcription regulators.Method With the signal molecule PhrG pentapeptide, RapG, and the transcription regulator DegU, a PhrG-RapG-DegU regulatory system was successfully constructed in B. subtilis 168 to fine-tune the expression of key genes, so that the expression of key genes in the MK-7 synthesis pathway can respond to the changes in cell density.Result The production of MK-7 in strain BS06 reached 102.47 mg/L through this system.Conclusion Fine tuning of quorum sensing can effectively improve the MK-7 production in B. subtilis 168.