Abstract:In order to screen probiotic lactic acid bacteria for food with cholesterol-lowering ability from fermented vegetable. In present study, taking Weissella confusa (RW) as a reference, 5 probiotic lactic acid bacteria isolated with cholesterol-lowering ability were obtained from different fermented vegetable samples via directional separation method combined with 16SrDNA identification method. The results showed that the five strains had excellent probiotic characteristics, with exhibiting the tolerance to acid(pH 2.0), bile salt(3 g/L) and simulated gastrointestinal fluids, and superior cholesterol-lowering ability(54.79%~62.54%)，and inhibiting pathogen and adhere to Caco-2 cells. The strains showed excellent probiotic characteristics. Further, five isolates were safety with no hemolysis phenomenon, no biogenic amines producing, and showed sensitivity to a variety of antibiotics. Five isolates were identified as Lactiplantibacillus plantarum (JCSMC-1), Lactobacillus plantarum (SYB and JCSMC6-2), Lacticaseibacillus paracasei (SYD and XSMC-1) by 16S rDNA. The isolates of 1 Lactiplantibacillus plantarum, 2 Lactobacillus plantarum, and 2 Lacticaseibacillus paracasei all have the basic characteristics of probiotic lactic acid bacteria for food, providing new resources for the development of probiotic lactic acid bacteria for food.
