Abstract:In order to explore the application of silver-loaded hydroxyapatite/titanium dioxide (Ag-HA/TiO 2 ) antibacterial agent in aquatic products, this study conducted the structural characterization of Ag-HA/TiO 2 and selected Escherichia coli and Staphylococcus aureus as the test strains for a comparison between silver-loaded hydroxyapatite(AgHAp) and titanium dioxide(Ag-HA/TiO 2 ) in antibacterial performance. A preliminary evaluation on the antibacterial rate of Ag-HA/TiO 2 against specific spoilage bacteria(SSOs) in aquatic products was also assessed. Based on these evaluations, the study conducted practical applications to investigate the effects of the antibacterial solution concentration and the soaking time on the microorganisms in large yellow croaker and the standard for Grade I fresh meat. The results showed that Ag-HA/TiO2 maintained its crystalline structure after loading titanium dioxide and reducing silver content by half. The antibacterial rate of Ag-HA/TiO 2 against Escherichia coli and Staphylococcus aureus was 100%, which was superior to AgHAp containing 3.00% of silver, and the minimum inhibitory concentration for both strains was 312.5 μg/mL. The antibacterial rates of 10 mg/mL Ag-HA/TiO 2 against SSOs-pseudomonas fluorescens and shewanella putrefaciens at 0.5 h were (97.58±3.43) % and 100% respectively, reaching 100% at 1 h and 1.5 h. As the Ag-HA/TiO 2 was applied to large yellow croaker, higher concentration of soaking solution or longer soaking time resulted in more pronounced inhibition effect on the growth of bacteria in large yellow croaker, extending the standard shelf life for Grade I fresh meat of large yellow croaker. The study demonstrates the high antibacterial effectiveness of Ag-HA/TiO 2 and suggests its potential as an ideal antibacterial agent for aquatic products.
