Abstract:Objective To provide a theoretical basis for the development and utilization of Actinomycetes resources in Baijiu-making environment,Actinomycetes were isolated and screened from high-temperature Daqu, and their functional characteristics were analyzed.Method Actinomycetes were screened from high-temperature Daqu using the dilution-plate coating method, and identified based on morphological characteristics and molecular biological evidence. Specifically, the metabolites of the strain were detected by high performance liquid chromatography and gas chromatography, and the Illumina platform was used for whole genome sequencing of the strain.Result The starch-degrading strain XJ-DQ-B-0001 was identified as Cellulosimicrobium funkei.The results of metabolite detection showed that strain XJ-DQ-B-0001 produced 14 metabolites, including lactic acid, acetic acid, β-phenyl ethanol, and ethyl acetate. The genome of strain XJ-DQ-B-0001 was 3 043 158 bp in length, with a GC percentage of 74.57% and 2 769 predicted protein-coding genes. The functional annotation of genes showed that the strain had the potential to produce carbohydrate hydrolases, flavor substances, and potential health factors.Conclusion The functional characterization of strain XJ-DQ-B-0001 shows that Actinomycetes can produce hydrolases, flavor substances, and health factors, which are beneficial to Baijiu brewing. The findings provide data support for the development and utilization of Actinomycetes resources in the Baijiu-producing environment.
