Development of ic-ELISA for Furosine Detection Based on Polyclonal Antibodies

Abstract:

A complete furosine complete antigen was prepared using the formaldehyde method, and cross-verification was employed to ensure the specificity of the polyclonal antibodies. An indirect competitive enzyme-linked immunosorbent assay(ic-ELISA) was successfully established based on the antibodies. The results demonstrated that the established ic-ELISA exhibited a detection range of 12.20 to 2 143.42 ng/mL, with a limit of detection (LOD) at 2.69 ng/mL. This method is simple, rapid, and cost-effective, offering high sensitivity and excellent specificity. It provides a novel option for the efficient detection of furosine in food, demonstrating its potential for practical application.