Abstract:To investigate the protective effect and mechanism of chlorogenic acid (CGA) on UVB damaged human immortalized epidermal cells (HaCaT), a cellular ultraviolet damage (photo-aging) model and different treatment methods of CGA were established using MTT assay. The UV absorption of CGA was detected by the microplate reader. The level of apoptotic cell death was determined by flow cytometry analysis. The free radical scavenging ability of CGA was detected by ABTS method. The results showed that when the UVB radiation dose was 21.6 mJ/cm2 and the concentrations of CGA were 100.000 and 150.000 μmol/L respectively, after pre-treatment for 1 hour and cell cultivation for 12 hours, CGA exhibited strong absorption in the UVB spectrum range of 280~320 nm. Both 100.000 μmol/L and 150.000 μmol/L CGA could significantly reduce UVB-induced cell apoptosis. The ABTS+ free radical scavenging capacity of UVB-treated cells could be effectively increased by 150.000 μmol/L CGA, reaching 53%. Therefore, CGA has strong anti-skin aging activity in the UVB-induced HaCaT photo-aging model, which might be related to its UV absorption and antioxidant ability. This provides a scientific basis for the development and utilization of food resources rich in CGA.
