Extraction Effect of Chitosan Modified Attapulgite on Nucleic Acid of Foodborne Pathogenic Bacteria

Abstract:

Surface modification of attapulgite (ATP) using chitosan was conducted to investigate the improvements in the adsorption performance of ATP, and a DNA extraction method based on chitosan-modified ATP was established to achieve low-cost and high-efficiency extraction of genomic DNA from foodborne pathogens. The material was characterized by X-ray diffraction spectroscopy and other techniques, while adsorption kinetics and adsorption isotherm models were used to investigate the adsorption mechanism of modified materials on DNA. Genomic DNA of three foodborne pathogenic bacteria was selected for extraction to investigate the extraction yield, purity, integrity, and stability. The results showed that the experimental data were well-described by the pseudo-first-order kinetic model and Freundlich model. Chitosan modification can significantly improve the adsorption performance of ATP. The saturation adsorption capacity of chitosan-ATP for herring sperm DNA achieved 23.648 μg/mg with high-quality and high-purity, and the chitosan-ATP based extraction method also showed good repeatability.