Components in Exudates from Threshing and Redrying Tobacco Leaves and Protective Effect of VC on EA.hy926 Cell Damage Induced by the Exudates

Abstract:

[Objective] This study aimed to explore the components in the exudates from threshing and redrying tobacco leaves and their effects on human vascular endothelial (EA.hy926) cell damage. [Method] The EA.hy926 cell model of cytotoxic damage was established with the exudates from threshing and redrying tobacco leaves. After pretreatment with VC, the methyl thiazolyl tetrazolium (MTT) method was employed to evaluate the viability of EA.hy926 cells, and the relative content of reactive oxygen species (ROS) in EA.hy926 cells was determined. [Result] Obvious cell damage was observed after 24 h of stimulation with the exudates from threshing and redrying tobacco leaves, which indicated the cell model of cytotoxic damage was successfully established. VC pretreatment significantly reversed the above changes. [Conclusion] VC exerts obvious protective effect on the cytotoxic damage of EA.hy926 cells that is induced by the exudates from threshing and redrying tobacco leaves by reducing the ROS level.