Effects of Glycosylation on the Binding of CBM to Cellulose and Lignin

Abstract:

[Objective] The aim of this study is to explore whether glycosylation affects the binding of the carbohydrate-binding module (CBM) to cellulose and lignin or not.[Method] Four CBM glycoforms CBM000, CBM010, CBM002, and CBM122 were prepared by chemical synthesis at three glycosylation sites Thr1, Ser3, and Ser14. Their binding abilities to cellulose and lignin at 4, 35, 45, 60, and 70 ℃ were compared. [Result] Glycosylation had different effects on the binding of CBM to cellulose and lignin at different temperatures. CBM000, CBM010, CBM002 and CBM122 have better selectivity for binding to cellulose at 45 ℃, 4 ℃/45 ℃, 60 ℃, and 45 ℃, respectively. [Conclusion] Rising the temperature can improve the substrate-binding selectivity of CBM. Glycosylation enhances the substrate-binding ability of CBM at high temperatures.