Abstract:To investigate the antioxidant activity of non-centrifugal sugar, tert-butyl hydroperoxide (t-BHP)-induced Caco-2 cells were used as the model of oxidative damage to assess the effects of non-centrifugal sugar crude extracts on cell viability, intracellular reactive oxygen species (ROS) levels, and cellular antioxidant activity (CAA). Additionally, high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was employed to analyze the main active phenolic compounds in non-centrifugal sugar. The results demonstrated that the non-centrifugal sugar extract could alleviate apoptosis induced by t-BHP, significantly reduce intracellular ROS levels, and exhibit notable antioxidant activity. The CAA value of the non-centrifugal sugar extract was equivalent to 12.27 μmol quercetin per gram of extract. Moreover, the non-centrifugal sugar extract contained phenols substances such as protocatechuic acid, vanillic acid, chlorogenic acid, cyanidin chloride, coumarin, and apigenin. This study provides a theoretical foundation for exploring the bioactivity of non-centrifugal sugar and its extracts, as well as the development of related functional products.
