Abstract:This study aims to evaluate the immunomodulatory function of Lactiplantibacillus plantarum (L. plantarum) CCFM8661 through animal experiments. A total of 120 male BALB/c mice were administrated with low, medium, and high doses of L. plantarum CCFM8661 or placebo by gavage for 30 d. The immunomodulatory capacity of L. plantarum CCFM8661 in mice was comprehensively evaluated by a series of experiments, including mouse splenic lymphocyte transformation and delayed type hypersensitivity (DTH) experiments to evaluate cellular immune function of the mice, serum hemolysin production assay and antibody-producing cell detection assay to assess humoral immune function of the mice, carbon clearance test and peritoneal macrophage swallowing fluorescent microspheres test to evaluate the monocyte-macrophage function of the mice, and natural killer (NK) cell activity assessment to evaluate the NK cell function of the mice. The results showed that L. plantarum CCFM8661 significantly enhanced the degree of ear swelling in the DTH response, increased the proliferation level of mouse spleen lymphocytes, promoted the production of serum hemolysin, and improved the phagocytosis capacity of peritoneal macrophages and carbon clearance capacity in the mice. These results indicated the capacity of L. plantarum CCFM8661 to enhance the immunity of mice, providing a theoretical basis for its practical application.
