Abstract:In order to evaluate the performance of colloidal gold rapid detection kit of aflatoxin B1 (AFB1), the quality of kits from six colloidal gold rapid detetion kit manufacturers was assessed. Blind samples were prepared by artificially spiked method using soybean oil as the matrix. The kit composition, pretreatment process, color development, performance indicators, and detection time of the products from six manufacturers were evaluated, and these kits were applied in the detection of edible oil samples in national and Henan province random inspections. The results showed that all product kits of six manufacturers had complete composition, fast and easy pretreatment processes, and short detection durations (all within 25 min). However, the pass rate was only 50%, with three kits failing due to high false-negative rates. Therefore, high-quality rapid detection kits can well meet the actual sample detection needs, however, the overall quality of rapid detection kits on the current market needs improvement. Manufacturers should strictly follow national standards for research and production. Meanwhile, relevant national departments should continue to improve the evaluation system and strengthen supervision.
