Preparation and Identification of 1-Pyrenebutyric Acid Artificial Antigen

Abstract:

The preparation and identification of 1-pyrenebutyric acid artificial antigen was investigated in this paper.1-pyrenebutyric acid was coupled to bovine serum albumin (BSA) and chicken oval albumin (OVA) using the carbodiimide method (EDC) to synthesize the artificial immunogen PBA-BSA and artificial detection antigen PBA-OVA.The results of UV scanning and polypropylene gel electrophoresis (SDS-PAGE) showed that the antigen was successfully prepared. The potency of mouse-derived polyantisera was more than 1 000 with IC₅₀ of 14.31 ng/mL. The cross-reactivity with polycyclic aromatic hydrocarbons 1-pyrene methanol, 1-pyrene formaldehyde and pyrene was less than 14.40%, and the cross-reactivity with phenanthrene, naphthalene, benzopyrene, BSA and OVA was less than 0.05%. PBA-BSA antigen was successfully prepared, and a sensitive murine multi antiserum was obtained, laying the foundation for the subsequent preparation of monoclonal antibodies and the establishment of rapid immunological detection methods.