Expression of Bone Morphogenetic Protein 10 and Its Mutants in CHO Cells

Abstract:

To address the issue of heterogeneous expression products derived from proBMP10 (the precursor protein of bone morphogenetic protein 10, BMP10) in Chinese hamster ovary(CHO) cells caused by Furin, the mutations of proBMP10 at its Furin recognition sites, proBMP10-1 (R313K) and proBMP10-2(R316K), were constructed and site-directedly integrated into the genome of CHO-K1-BAK^-/BAX^- respectively. The recombinant CHO cell lines stably expressing the target proteins were successfully established. The results showed that the proBMP10-2 (R316K) was no longer cleaved by Furin and retained biological activity, while proBMP10-1 (R313K) continued to be cleaved by Furin.