Effect of Wall Breaking Methods on Antibacterial Activity of Sexual Propagator of Eurotium cristatum

Abstract:

To investigate the effect of three wall breaking methods on the antibacterial properties of sexual propagule (ascocarp and ascospore) extracts of Eurotium cristatum, ultrasonic method, germination method and glass bead-beating method were used to prepare the aqueous extracts. The antibacterial activities of extracts against Staphylococcus aureus and Escherichia coli were determined by the disk diffusion test. The results suggested that the ascocarps were much more fragile and their aqueous extracts prepared by glass bead-beating method displayed stronger antibacterial activity than those of ascospores. The radius of inhibitory zone of ascocarp extracts against Escherichia coli and Staphylococcus aureus were 5.8 mm and 12.6 mm, respectively. The highest spore breakage rate was 94.8% when glass bead-beating method was applied to disrupt ascospores and the obtained extracts displayed strong antibacterial activity. However, the extracts obtained by the other two methods showed insignificant antibacterial activities. Therefore, glass bead-beating method was the best method to extract the antibacterial substances from Eurotium cristatum sexual propagules among the three methods.