Screening, Isolation and Identification of κ -Carrageenan Lyase-Producing Bacteria

Abstract:

Using κ -carrageenase as the sole carbon source, 4 strains with κ -carrageenase activity were screened from 13 samples of sea mud with different coordinates in Dalian, Liaoning Province. The DNS method (3,5-dinitrosalicylic acid method) was then used to detect the κ -carrageenase activity produced by 4 strains. According to the κ -carrageenase activity comparison, a high-yielding strain was named LG-2, with a maximum enzyme activity of 34 U/mL. Based on morphological observation, physiological and biochemical identification, and 16S rDNA phylogenetic tree analysis, the strain was initially identified as Rhodococcus and Gram-positive bacteria. The growth curve and enzyme activity curve of LG-2 strain were determined, laying the foundation for industrial production.