Abstract:An ultra performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) method was established for the simultaneous determination of 20 kinds of veterinary drug residues of β-receptor activitors, sulfonamides, sulfone inhibitors, quinolones, glucocorticoids and macrolides in porcine muscle. The drugs were extracted with 80% acetonitrile, purified by the solid-phase extraction cartridge, dissolved with 10% methanol after nitrogen blowing, and detected by UPLC-MS/MS after filtering by microfiltration membrane. The BEH C18 column was used for the separation by gradient elution. The identification was achieved by electrospray ionization in positive mode and multiple reaction monitoring. The limit of detection(LOD) was from 0.5 to 5 μg/kg and the limit of quantitation(LOQ) was from 1.7 to 16.7 μg/kg, the recovery rate range was between 62.1%~118.3% and the relative standard deviation(RSD) was 0.4%~16.7% when the added standard substance concentration was 1, 5, 10 μg/kg.
