Abstract:This article aimed to study the anti-inflammatory mechanism of Tricholoma mongolicum polysaccharide extract in LPS-treated RAW264.7 cells. The RAW264.7 cells were first treated with different concentrations of Tricholoma mongolicum polysaccharides, and the viability was determined by CCK-8. The release of NO was measured by Griess, and TNF-alpha and IL-1 beta were examined by ELISA. The expression levels of HIF-1α，NF-κB，STAT3，COX-2 and iNOS mRNAs and proteins were tested by qPCR and western blot. The results showed that the maximum safety dose of Tricholoma mongolicum polysaccharide extract was less than 100 μg/mL. Compared with the LPS model group, Tricholoma mongolicum polysaccharide extract at the concentrations of 0.01, 0.1, 1, 10 μg/mL could inhibit the production of NO,TNF-α and IL-1β. The inhibition rates of NO were 38.21%, 64.17%, 58.16%, 79.42%, respectively; the inhibition rates of TNF-α were 27.91%, 36.31%, 36.14%, 45.09%, respectively; the inhibition rates of IL-1?茁 were 47.75%, 58.47%, 60.21%, 64.55%, respectively. Tricholoma mongolicum polysaccharide extract at the concentration of 10 ?滋g/mL could down-regulate the mRNAs and proteins expression levels of HIF-1α, iNOS, STAT3 and COX-2. Therefore, anti-inflammatory effect of wild Tricholoma mongolicum polysaccharides on LPS-induced RAW264.7 cells may be exerted via STAT3 and HIF-1α pathways.
