Abstract:The gene cluster for pyrroquinoline quinone (PQQ) synthesis was cloned from Pseudomonas plecoglossicida JUIM01. Its genome composition and biological information were subsequently analyzed. According to the reported genome sequence of P. plecoglossicida JUIM01, primers were designed for amplification of the PQQ synthesis gene cluster via LA-PCR. The gene fragments were sequenced and analyzed by bioinformatics approaches. The results showed that the total length of the cloned gene fragment was 11 659 bp, which included 9 genes: pqqF, pqqA, pqqB, pqqC, pqqD, pqqE, pqqM, pqqH and pqqI, and encoded the related enzymes in PQQ biosynthesis pathway and precursor peptide PqqA. These encoding genes were similar to the PQQ synthesis gene cluster of P. fluorescens Pf0-1 with sequence identifity of 41~94%. This study, for the first time, reported cloning of the PQQ synthesis gene cluster from P. plecoglossicida JUIM01, and the bioinformatics analysis laid a foundation for the study of PQQ biosynthesis pathway and intracellular regeneration mechanism of Pseudomonas strains. Furthermore, it provided theoretical support for improving the production intensity of 2KGA.
