Screening and Identification of A High-Efficiency Penicillin Sodium-Degrading Strain

Abstract:

Strains tolerant of 20 g/L penicillin sodium were screened from the soil near the penicillin production company. A strain named Anti-Pen20 with highly efficient degradation of penicillin sodium was screened by high performance liquid chromatography. Based on the colony, cell morphological characteristics and the analysis of the 18S rRNA gene sequence, the strain Anti-Pen20 was found to belong to the genus Rhodotorula mucilaginosa. The degradation rate of penicillin sodium was 85% after culturing 24 h at 30 ℃ when Anti-Pen20 was inoculated with 15% and the initial concentration of sodium penicillin was 20 g/L. Anti-Pen20 showed high degradation rate in high concentration penicillin sodium medium, which is expected to be applied to the recovery of high concentration penicillin sodium slag, also provides research basis for the application of Anti-Pen20 as an additive for disease prevention in animal feed and fruit industry.