Abstract:This study aimed to investigate the difference of immunomodulatory activity between polysaccharides from fresh (LPX3) and dried longan pulp (LPG2). The effects of LPX3 and LPG2 on the proliferation of mouse mesenteric lymph node cells and macrophages, phagocytosis and NO, IL-6 and TNF-α secretion of macrophages were analyzed. The results showed that LPX3 and LPG2 significantly promoted the proliferation of mouse mesenteric lymph node cells and macrophages, and promoted the macrophage phagocytosis and NO secretion(P< 0.05). When treated with 400 μg/mL LPX3 or LPG2, the secretion levels of IL-6 were increased to 27.31 times and 31.54 times of the control, respectively, while TNF-α contents were increased to 17.67 times and 18.42 times of the control, respectively. At the same concentration, LPG2 exhibited a stronger effect on promoting the proliferation of mesenteric lymph node cells and macrophages and activating macrophages.
