Quantitative Analysis of Six Soybean Isoflavones by QAMS

Abstract:

The study aimed to establish a method for quantitative analysis of multi-components by single-marker (QAMS) to quantify six soybean isoflavones by HPLC with ultraviolet detection of dual wavelength，which would decrease the cost of quantitative analysis of isoflavones and enhance the efficiency. The sample was extracted by methanol under the ultrasonic wave assistance，then analyzed by HPLC with a C18 column. The running condition was 25 ℃，injection volume of 10 μL，detection wavelength of 262 nm and 248 nm，elution rate of 1.0 mL/min with 0.2% formic acid in water and methanol. Six soybean isoflavones were used for the QAMS to obtain the standard curves，linear ranges，relative correction factors (RCF)，relative retention times，and relative peak areas. The precision，stability，repeatability，sample recovery rate，system durability and adaptability of QAMS method were tested and compared with those of external standard method. The results showed that six soybean isoflavones had good linearity within the analysis range (R² > 0.999 9).