A Review on Application of Nucleic Acid Hybridization-Mediated Fluorescence Signal Amplification in Biological Detection

Abstract:

Nucleic acids hybridization mediated fluorescence signal amplification methods refer to a kind of methods of triggering or blocking the hybridization of nucleic acids by targets, thus causing changes in the number of labeled fluorescence on the hybridization products. Because of flexible design，good specificity，high sensitivity，short response time and low background signal，these methods have been widely used in biological detection. This article reviewed the applications of these methods in the detection of bacteria，nucleic acids，proteins，biological small molecules and inorganic metal ions，as well as their problems and development tendency.