Construction and Panning of Single Domain Antibody Library against Silver Carp Myofibrill-Bound Serine Protease

Abstract:

Myofibril-bound serine proteinase（MBSP）in fish can degrade myofibrils during processing，resulting in gel deterioration of surimi products. A new highly specific shark-derived antibody，single-domain antibodies（sdAbs)，binds directly to antigens and inhibits enzyme activity. In this paper, nurse shark (Ginglymostoma cirratum) was immunized with expressed MBSP to construct the library of single domain antibody, and 31 phagemids specific to MBSP were panned from the library. The results could provide the basis for the expression and identification of specific single domain antibody which was expected to overcome the gel deterioration of the surimi products by inhibiting or eliminating the activity of MBSP.