Improving Fructosylated Chondroitin Production by Enhancement of UDP-GalNAc Synthetic Pathway

Abstract:

Sulfated chondroitin is an important glycosaminoglycans，which is widely used in medical，food and cosmetic fields. To improve the production of fructosylated chondroitin，GlcN synthase（GlmM） and GlcN-6-P synthase（GlmS） were overexpressed，the confusion protein was constructed and a recombinant strain E. coli K4-H-glmM-glmS was obtained. Then，the optimal induction conditions including IPTG concentration and induction temperature were estalished. Finally，with the DO-stat feeding strategy in a 5 L fermenter，the yield was up to 3.99 g/L，which was 108.90% higher than that with wild type strain. The study provides a basis for industrial-scale production of fructosylated chondroitin.