Physiological Characteristics，Safety Evaluation and Genetic Analysis of Bifidobacterium longum CCFM760

Abstract:

Bifidobacterium longum CCFM760 is screened from stools of a healthy two-year-old youngster in Binhu District，Wuxi City，Jiangsu Province，China. In this study，the ability to grow and produce acid，artificial simulated gastrointestinal fluid tolerance，antibacterial ability，oligosaccharide utilization ability and antibiotic tolerance of B. longum CCFM760 were assessed，as well as its total superoxide dismutase (SOD) activity. It was found that this bacterium had good growth acidogenicity and artificial simulated gastrointestinal fluid tolerance，and obviously inhibited Escherichia coli and Staphylococcus aureus. Meanwhile，it could significantly utilize seven oligosaccharides and was sensitive to 14 antibiotics. The total activity of SOD in the culture reached（99.14±9.91）U/mL. In addition，the genome sequence of the strain was sequenced to explain the ability of oligosaccharide metabolism，antibiotic sensitivity and anti-oxidation of the bacteria. These results demonstrated that B. longum CCFM760 is a safe and potential probiotic.