Optimization of Medium by Response Surface Methodology for Vitamin C Mixed Culture Fermentation with A New Companion Strain Rhodotorula mucilaginosa

Abstract:

A new company strain of A8, which can promote 2-keto-L-gulonic acid（2-KGA） produced by K. vulgare in vitamin C（VC）mixed culture fermentation，was separated from the source of Longwei Lake silt，Dongling Shenyang. The ITS rDNA was amplified by PCR and sequenced to obtain a 610 bp ITS rDNA. Analysis was performed by homology comparison with the NCBI database to construct the phylogenetic tree basing on ITS rDNA sequences. And the strain of A8 was determined as Rhodotorula mucilaginosa based on the molecular identification combining with morphological identification. The R. mucilaginosa and K. vulgare composed of a new mixed culture of VC fermentation system and the production yield of 2-KGA was 52.56 g/L.