Preparation of Cardamine Protein and Its Antioxidant Activity

Abstract:

In order to develop and utilize Enshi Cardamine，in this paper，the extraction process of selenoprotein was studied，and the antioxidant activity of selenoprotein was investigated. The results indicated that the optimal conditions for the extraction were as follows: material ratio 1:40 g/mL，the extraction temperature was 50 ℃，the extraction time was 8 h by distilled water and then 8 h by 0.1 mol/L NaOH solution. Under the optimal conditions，the extraction rate of selenopratein was up to 75.4%. The content of water soluble protein was 26.5 g/dg，the selenium content was 1 748 μg/g，the content of alkali soluble protein was 52.3 g/dg，and the selenium content was 1 513 μg/g. The alkali soluble protein were separated by Sephadex G-100 gel chromatography，and two components were obtained，peak 1 and peak 2. The selenium content of peak 1 was 668 μg/g and the selenium content of peak 2 was 650 μg/g. By studying the ability of cardamine proteins to scavenge hydroxyl free radicals（·OH）and 1-diphenyl-2-trinitrophenyl hydrazine free radicals（·DPPH)，it was found that the cardamine protein have certain ability to scavenge free radicals.