Deproteinization Technology by Enzymic Method of Polysaccharide from Yam Mucilage and Its Performance Study

Abstract:

The response surface methodology was used to optimize the deproteinization technology of yam mucilage polysaccharide. The results indicated that the optimal conditions were pH 8.0，time 2 h，liquid-solid ratio 80 mL/g，enzyme-substrate ratio 30% and temperature 37 ℃. Under these conditions，the protein removal rate and polysaccharide retention rate were 83.4% and 92.7%，respectively. Through testing the free-radical scavenging rates of yam mucilage polysaccharide，the results showed that yam mucilage polysaccharide's abilities of scavenging DPPH free radicals and hydrogen peroxide were very strong，and the clearing rates could achieve 80% above；but its ability of scavenging OH free radical was weak. In 43% and 81% relative humidity，by testing the moisture absorption rate and moisture rate，the results showed that the comprehensive moisturizing property of yam mucilage polysaccharide was slightly better than that of sodium hyaluronate.