Abstract:The effects of soybean peptides from soybean protein isolate(SPI) hydrolyzed by the recombinant Aspergillus sojae alkaline protease (rAp) on immunity function and antioxidationin mice was explored. Soybean protein isolate (SPI) was hydrolyzed by induced expression of purified rAp, and then the hydrolysate was administered to mice. The effects of its hydrolysate on immune organ index, serum lysozyme activity and serum anti-bacterial activity of mice were determined. The antioxidant activity of GSH-PX and CAT, and the content of MDA in mice were measured based on the anti-oxidation test carried out in mice. Furthermore, the effect of soybean peptides on the activity of GSH-PX, CAT and the content of MDA in mice blood were determined. The results indicated that soybean peptide had no significant effect on immune organ index of mice, but it had significant improvement on serum lysozyme activity and serum anti-bacterial activity of mice. It had no significant effect on serum GSH-PX activity of mice, however, CAT activity and MDA content in serum were significantly increased. In conclusion, the soy peptides hydrolyzed by rAp could enhance the immune function and antioxidant activity of mice, providing experimental basis for high value utilization of soybean in health food industry.
