Effect of Two Kinds of Polysaccharide on Immune Performance of Schizothorax prenanti

Abstract:

The experiment was conducted to investigate the effects of oxidized konjac glucomannan and oxidized konjac glucomannan sulfates on immune performance of Schizothorax prenanti. The Schizothorax prenanti were randomly divided into 7 groups and each group included 3 replicates of 20 fish. The experiment lasted for 60 days with the treatment groups were contained 0.4%、0.8%、1.6% of OKGM and OKGMS. And body composition and serum biochemical indices were measured and MyD88、IRF7 mRNA level in tissue were determined by RT-PCR. Results：Content of IgM、ACH50 of OKGMS1.6 were significantly more than the control group；The content of MDA was significantly lower of group OKGMS0.4、OKGMS0.8、OKGM0.4、OKGM0.8 than the control group；OKGMS、OKGM both increased the activity of SOD；OKGMS could up-regulate MyD88 mRNA level in gut and IRF7 mRNA level in liver、spleen and mesonephros；OKGM could increase MyD88 mRNA level in gut、headkidney and mesonephros and IRF7 mRNA level in gut、liver、spleen and mesonephros. Conclusions：Add the appropriate amount of OKGM and OKGMS could increase the antioxidant activity and immune functions of Schizothorax prenanti.