Stabilization of Porcine Pancreas Lipase using a Solubility Reversible Polymer Eudragit S-100

Abstract:

In order to avoid the bad effect on enzyme catalytic efficiency and reuse with traditional insoluble carrier，a new kind of smart polymer named Eudragit S-100 was used for the immobilization of porcine pancreas lipase（PPL）. Eudragit S-100 is an anionic copolymer of methacrylic acid and methylmethacrylate，and has a solubility reversible. The results showed that the modification rates of free amino of PPL immobilized with Eudragit S-100 reached 52.4% at 15 ℃ after 6 hours. The fluorescence emission spectra indicated that the enzyme molecule peptide chains were fold，and finally the structure became compact. The optimum temperature of immobilized PPL did not change，but the optimum pH moved slightly to an alkaline direction. Eudragit S-100 immobilization enhanced the enzyme stability against changes of pH and temperature. Furthermore，the immobilized PPL gained the solubility reversible on the basis of pH，and they had a high reusability，retaining about 45% of its original activity after five cycles of repeated uses.