Co-Effect Cytotoxicity and Mechansim Research of Deoxynivalenol and Aflatoxin B1 in HepG2/C3A Cells

Abstract:

Deoxynivalenol（DON） and Aflatoxin B1 are the most prevalent mycotoxins in cereal-based ingredients or foods and they frequently coexist. HepG2/C3A cell treated with different concentrations of DON（0.56、1.125、2.25、4，5、6.75 ?滋mol/L） and AFB1（2.5、5、10、20、30 ?滋mol/L） for 24 h was used to analyze their toxic effects expressed by cell proliferation inhibition rate（SRB），total double-stranded DNA，apoptosis rate，cell cycle arrest and apoptosis-related gene，caspase-3，Bax，Bcl-2 were analyzed by real-time quantitative PCR .The results showed that their co-effect was classified as being additive. Flow cytometry assay on the rate of apoptosis also shown an additive interaction which could indicated that these two toxins could induced apoptosis and they also induced cycle arrest in S-phase or S，G2/M- phase. The increase of expression of caspase-3 and Bax as well as the decrease of Bcl-2 further support their additive toxic effect. RNA-Seq analysis showed different cytotoxicity mechanism between AFB1 and DON that AFB1 and their combination effect induce cell apoptosis mainly through P53 pathway while not for DON. We guess AFB1 and DON may through different pathway to induce cell apoptosis together.