Separation，Purification and Structural Identification of Iron-Chelating Black-Bone Silky Fowl Oligopeptides

Abstract:

In order to investigate the structure of peptides fromiron-chelating black-bone silky fowloligopeptides （BSFP-Fe），BSFP-Fe wasprepared by using black-bone silky fowl as raw material andiron chloride as iron resource. The total protein content，chelating rate and yield were determined. Results showed that the total protein content，chelating rate and yield were 56.79±0.18%，83.53±0.15% and 40.86±0.14%，respectively. Then，BSFP-Fe was separated and purified by reversed phase high performance liquid chromatography（RP-HPLC）.A total of4 fractions were collected and subjected to Q-TOF mass spectrometer to identify peptide sequences. One peptide，Thr-Ser-Gly-Met- Pro，was identified and its molecular weight was 491.56 u. This studyconfirmed the structure of peptides from BSFP-Feand provided certain theoretical support for the development of BSFP-Fe as iron supplement food.