Isolation，Purification and Physicochemical Properties of Polysaccharides from Chinese Rice Wine

Abstract:

In this study，Chinese rice wine from Shaoxing region was used to extract polysaccharides，and the protein of crude polysaccharides sample was precipitated by sevag. The isolation，purification of polysaccharides was performed by DEAE-Sepharose Fast Flow and Sephadex G75. Furthermore，the purity and molecular weight were determined by high performance gel permeation chromatography （HPGPC）. Ultraviolet spectrum （UV），fourier transform infrared spectrometry （FTIR） and NMR were for determining the structure of CRWP1. It was showed as a single symmetrical peak on high-performance gel-permeation chromatography （HPGPC） and the average molecular weight was 7 850 Da. The results showed that CRWP1 was a heteroglycan，which was composed mainly of glucose，arabinose，xylose，and a small quantity of fucose，galactose and mannose. There was one glycosidic configuration （α） in structure of polysaccharides from Shaoxing rice wine，and the glycosidic bond was galactopyranose type. It was also revealed that the structure of polysaccharides from Shaoxing rice wine contained 1，3 glycosidic bonds as the main chain and 1，4 glycosidic bonds as branched chain.