Optimization of Multi-Enzymatic Extraction Process of Polysaccharide from Ploygonatum sibiricum

Abstract:

Method of multi-enzyme was used to optimize the extraction process of polysaccharide from Ploygonatum sibiricum，phenol-sulfuric acid colorimetric method was applied to determine the content of polysaccharide，defined the polysaccharide extraction rate as the index to choice the kind and ratio of enzymes，the experimental factors were selected by single factor trials such as enzymatic hydrolysis temperature，pH，the rate of material to solvent，the amount of enzyme concentration，the important ones of which would be chosen to optimize by orthogonal test. Results showed that multi-enzyme extraction was better than extraction by single-enzyme or water. The rate of cellulose to papain was 3 to 7. The optimum enzymolysis conditions were as following：pH value of 5.0，temperature of 50 ℃，the rate of material to solvent was 1 to 20，the amount of enzyme concentration was 1.5%，which was 0.45% of cellulose and 1.05% of papain，2 hour-hot water extraction after 2 hour-enzymatic hydrolysis. In this condition，the maximum polysaccharide rate was 21.55%，which was 2.75 times as high as water，and had 12.6% higher than that of single factor trials.