Isolation，Identification and Antibacterial Effect by Tannic Acid of Thermoacidiphilic Bacteria From Citrus

Abstract:

In order to provide methods to provent Thermoacidiphilic Bacteria From juice，we study the characteristics of Thermoacidiphilic Bacteria from citrus.The Thermoacidiphilic Bacteria which was isolated from the citrus was investigated by comparison with the standard strain using Kirin-medium acidified with malic acid. Different concentrations of tannic acid were tested in Thermoacidiphilic Bacteria from citrus. The minimal inhibitory concentration was measured through the agar dilution. The results shows the two polluted bacteria isolated from citrus can grow under the temperature of 25~55 ℃ and pH of 2.4~6.0，which was corresponded with the characteristics of the Thermoacidiphilic Bacteria. Further more，the cell morphology，colony morphology，cultural characteristics，physiological characteristics and 16S rDNA tests indicated the two isolated strains of Thermoacidiphilic Bacteria have obviously similar characteristics with the standard strain. The results showed that tannins had good antibacterial activities on thermoacidiphilic bacteria，MIC of which is 1.25 mg/mL. Isolates show a high degree of similarity with the standard strains.