Process Optimization of the Ultra-High Pressure Extraction of Hemoglobin from Chicken Blood by Response Surface Methodology

Abstract:

This study was under taken to exploring the optimal process for ultra-high pressure extraction of chicken blood hemoglobin（Hb） and demonstrating the key process properties. To accomplish this，response surface analysis methodology（RSM） was used for optimizing ultra-high pressure extraction of Hb with Hb rate as the response. In the meanwhile，the role of ultra-high pressure in improving Hb functional properties by high speed homogenization，such as emulsifying properties and foaming capacity，were also revealed. Results showed that the technical parameters，including liquid ratio，extraction time and ultra-high pressure presented significant impact on Hb yield. The optimal extraction conditions were 1 g∶250 mL solid-liquid ratio，135 MPa for 5.4 min. Under these conditions，the yield of Hb was 6.697 g/dL. With ultra-high pressure treated，the emulsifying property of Hb was obviously improved while its emulsion stability decreased；both the foaming capacity and foaming stability presented enhanced trends.