Preparation and Antibacterial Activities of Dextran-Amphotericin B Conjugates

Abstract:

Three oxidation degree of dextran derivatives（D-CHO） were synthesized with oxidation of sodium periodate. Dextran-amphotericin B（D-CHO-AmB） conjugate was prepared by Schiff's base reaction between aldehyde groups of D-CHO and amino groups of AmB. MALS/GPC results indicated that the molecular weight of dextran was reduced with the increase of oxidation degree. The drug loadings of D-CHO-AmB were 18.9% to 36.7%，which increased with the oxidation degree. Compared with AmB injection，the hemolytic effect of D-CHO-AmB was decreased significantly and the critical concentration of hemolysis was almost tenfold. The inhibition zone test proved that D-CHO-AmB conjugates had an excellent antifungal effect.