Determination of Entrapment Efficiency of Beta-Ionone Liposomes

Abstract:

A method used for determining the entrapment efficiency of beta-ionone in liposomes was established. The liposome was prepared by thin film evaporation. The free beta-ionone was separated from liposomes by reverse dialysis and quantified with HPLC. The results showed that the calibrated curve was linear（R2=0.999 8） within the range of 5~80 mg/L for beta-ionone. Intra-day and inter-day precision（RSD）were both less than 3%. The equilibrium time for the dialysis was 12 h， and the recoveries showed with good reproducibility（99.21%~101.22%），and the leaking percentage in 20 h was negligible. The average entrapment efficiency of beta-ionone liposome was 52.76±1.10%. The reverse dialysis method was convenient，accurate and economic，suitable for determining the encapsulation efficiency of beta-ionone liposomes.