Relationship between Mycelium Morphology and Intracellular Triterpenoids Production of Poria cocos in Submerged Culture

Abstract:

This research aims at investigating the effects of fermentation conditions on mycelium morphology and total triterpenoids yield during submerged culture of Poria cocos. The results indicated that the total triterpenoids main derived from middle-sized equivalent diameter and pellet in smooth shape were more conducive to enhancing the total triterpenoids production. The appropriate condition of submerged culture were broth content 50 mL，inoculate size 6%，pH 5.5，temperature 26 ℃，rotation speed 130 r/min for 11 days. The positive correlations both production of total triterpenoids and M diameter growth as well as smooth pellet have shown that the relationship between mycelium morphology and intracellular triterpenoids.