Effect of Sucrose on Qiweibaishusan to Intestinal Bacteria Diversity in Dysbacteriotic Diarrhea Mice

Abstract:

In order to evaluate the effect of sucrose on Qiweibaizhusan to intestinal bacteria diversity in dysbacteriotic diarrhea mice，48 SPF mice were randomized into 4 groups. Normal control group of mice were via intragastric administration of sterile water. Model group and treated groupmice were constructed via intragastric administration of cefradine and gentamycin sulfate. Model group mice were via intragastric administration of sterile water，and treatment groups were via intragastric administration of 50% amount of ultra- micro Qiweibaizhusan powder and 50% amount of ultra-micro Qiweibaizhusan powder with 8% sugar respectively. After treatment，intestinal microbiotia metagenome DNA were extracted. The intestinal bacteria diversity was analyzed by amplified ribosomal DNA restriction analysis. The results showed that OTU and stripe numbers in 50% amount of ultra-micro powder Qiweibaizhusan group，50% amount of ultra-micro powder Qiweibaizhusan with sucrose group and normal control group were all 6. The diversity index in 50% amount of ultra-micro powder Qiweibaizhusan group，50% amount of ultra-micro powder Qiweibaizhusan with sucrose group and normal control group were respectively 2.322，2.585 and 2.585. The same similarity coefficient in 50% amount of ultra-micro powder Qiweibaizhusan group，50% amount of ultra-micro powder Qiweibaizhusan with sucrose group and normal control group were respectively 0.363 6，0.277 8 and 1.0000. The similarity coefficient in 50% amount of ultra-micro powder Qiweibaizhusan with sucrose group is lower than that in 50% amount of ultra-micro powder Qiweibaizhusan group. Cluster analysis and principal component analysis showed that 50% amount of ultra-micro powder Qiweibaizhusan group were better than 50% amount of ultra-micro powder Qiweibaizhusan with sucrose group to normal control group. Both 50% amount of ultra-micro powder Qiweibaizhusan and 50% amount of ultra-micro powder Qiweibaizhusan with sucrose could cure dysbacteriosis diarrhea. The former had better effect than the latter. It was advised to adopt the ultra-micro powder Qiweibaizhusan without the additive of sucrose.