Enzymatic Synthesis of L-2-Methyltryptophan Catalyzed by Tryptophan Synthase in a Water/Organic Solvent Biphase System

Abstract:

L-2-methyltryptophan plays an important role in many physiological processes. This paper investigated the synthesis of L-2-methyltryptophan in an aqueous/organic biphasic system，and optimized the synthetic method. The optimal transformation conditions were 150 mmol/L L-serine，2% toluene，0.1 mmol/L Ca2+，2% Trion X-100，40 ℃ and pH 8，which resulted in a content of over 21.17 g/L for L-2-methyltryptophan. Thus，the strain can be used to produce L-2-methyltryptophan and has an excellent converting capability of L-2-methyltryptophan.