Effect of High Hydrostatic Pressure Treatment on the Enzyme Activity of Lipase Lip2 from Yarrowia lipolytica

Abstract:

The study investigated the effects of pressure，temperature，dwell time and pH on the enzyme activity of lipase Lip2 from Yarrowia lipolytica（YLLIP2） in high hydrostatic pressure（HHP） treatment. Results showed that HHP improved the activity of YLLIP2 and pressure，temperature，dwell time and pH had significant effects on the enzyme activity. Optimal parameters were found by orthogonal test to be pressure 450 MPa，temperature 45 ℃，dwell time 10 min and pH 7.5. The optimal pH was increased 0.5 in HHP.