Fast Quantitative Analysis of Lipids and DHA Contents During Fermentation Process for DHA Production

Abstract:

A simple，fast and accurate method was established to determine the composition and contents of lipids and docosa-hexaenoic acid（DHA） during fermentation process for DHA production. The detection for lipids and DHA contents in Schizochytrium was optimized by drying methods，cell disruption methods，the ways of lipid extraction，the methods of methyl esterification and the selection of internal standards. The optimal analysis used an acid-heating method for cell disruption，n-hexane for lipid extraction，nonadecanoic acid methylester as the internal standard and methyl esterification catalyzed by alkali catalyst. It was particularly suitable for the intermediate analysis during fermentation process for DNA production which could be accomplished within 1-2 hours.